Name: Stuart EdwardsTopic: The effect of pH and temperature on the catalase enzyme.A research assignment: Proposal Draft 2St. Stithians Boys CollegeContentRationale Pg. 2Conceptual diagram Pg.2Literature review Pg.3Topic Pg. 4Methodology Page 5• Hypothesis Page 5 5• Objective Page 5• List of devices Pg. 5• Method Page 6• Data collection Page 7• Validity and reliability page. 8• Presentation and analysis of data Pg. 8Results Pg.9Graphs Pg. 10Analysis and conclusion of the question asked page. 12Appendix Page 14...... in the center of the paper ......and substances at 37.5̊C because in the previous experiment it was discovered that this is the optimal temperature at which catalase reacts. It was because of this constant that I used the 37.5°C catalase data set from the first experiment to provide a neutral environment for the experiment. The way data was collected for the first experiment was identical to that needed for the second. From this data it was determined that the neutral environment for catalase had the best results, which makes it clear that when the enzyme is at opposite extremes of pH such as basic or acidic, it is unable to function properly. When it is too basic the enzyme will become inactive and when the enzyme is too acidic the enzyme will denature, making it unable to function at the optimal efficiency that all enzymes need.